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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already explained. Two added splice variants ended up documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guide outlines further doable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This guide outlines additional possible optimization of cellular and immunoassay parameters to ensure the absolute best effects are...
Antibodies bound to the blocking peptide no longer bind on the epitope over the goal protein. By evaluating the staining with the blocked antibody as opposed to the antibody alone, you can see which staining is certain.
Target Facts Cyclin E belongs for the hugely conserved cyclin loved ones, whose members are characterised by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which add towards the temporal coordination of each and every mitotic event. Cyclin E varieties a complex with and capabilities like a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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